This is a definite recommendation for any molecular biologist. The analysis of haplotypes from DNA sequences has been improved: haploNet now returns alternative links, and there is a new function, replot, to help plot. The CRISPR/Cas9 system is very effective at inducing site-specific indels in zebrafish (Chang et al., 2013 Hwang et al., 2013).A mix of site-specific CRISPR sgRNA and Cas9 protein is injected into 1-cell stage zebrafish embryos to create indels at the target site, resulting in a pool of F 0 embryos that are genetically mosaic. The website for ApE is nothing flashy or fancy (and for that matter, neither is the program itself), but it is fully functional and does the job well. Users can perform “virtual digests” whereby they select a predefined DNA ladder, or specify their own, and visualize the theoretical DNA fragments. ApE is a sequence editor, and contains powerful general and DNA specific text editing tools, including basic text input, sequence search, ORF search, specialized copy and paste functions, and brief instantaneous analysis of selected text. The Chimpanzee Sequencing and Analysis Consortium Initial sequence of the chimpanzee genome and comparison with the human genome. New analysis of the DNA sequence of the male-specific Y chromosomes from all living species of the great ape family helps to clarify our understanding of how this enigmatic chromosome evolved. What is the trend in the number ape-like and human-like traits when comparing older hominid fossils to more recent ones 2. There is a feature to display the translation of the coding region, as well an option to identify and display restriction enzyme sites within the DNA. By continuing to use this site, you agree to the use of cookies. Skip to main content This website uses cookies to ensure you get the best experience.
Within the program, users can view circular or linear maps of their DNA sequences. Students will also begin computer-based DNA sequence analysis of the TAS2R38 gene during this incubation period. In ApE in order to align sequences you should first open all the files and choose the reference sequence as well as the Align to Windows in the Align DNA. The Addgene analyze sequence program is a tool for basic DNA sequence analysis that can detect common plasmid features in the sequence and create a map from those features.
ApE is free to download and runs across all three major platforms: Windows, OS X, and Linux/Unix. Strong Points: Easy-to-use program, works across platformsĭo you drive yourself bananas trying to find a free, easy-to-use program to analyze plasmids, restriction digests, etc.? Well, you’ll go “ApE” over A Plasmid Editor, a program as simple in use as it is in name.